|
column 1
item |
column 2
class |
column 3
host |
column 4
vector |
|---|
| 1 | bacteria | Escherichia coli K12, E. coli B, E.
coli C or E. coli Nissle 1917—any derivative that does not
contain— (a) generalised transducing phages; or (b)
genes able to complement the conjugation defect in a
non-conjugative plasmid | 1 non-conjugative plasmids 2
bacteriophage— (a) lambda (b)
lambdoid (c) Fd or F1 (eg M13) 3 none
(non-vector systems) |
|
| Bacillus —specified
species—asporogenic strains with a reversion frequency of less than 10
–7 : (a) B. amyloliquefaciens (b) B.
licheniformis (c) B. pumilus (d) B. subtilis
(e) B. thuringiensis | 1 non-conjugative plasmids
2 plasmids and phages whose host range does not include B.
cereus , B. anthracis or any other pathogenic strain of Bacillus 3
none (non-vector systems) |
|
| Pseudomonas putida
—strain KT 2440 | 1 non-conjugative plasmids
including certified plasmids: pKT 262, pKT 263, pKT 264 2
none (non-vector systems) |
|
| Streptomyces —specified species: (a)
S. aureofaciens (b) S. coelicolor (c)
S. cyaneus (d) S. griseus (e) S. lividans
(f) S. parvulus (g) S. rimosus (h)
S. venezuelae | 1 non-conjugative plasmids 2
certified plasmids: SCP2, SLP1, SLP2, PIJ101 and derivatives 3
actinophage phi C31 and erivatives 4 none (non-vector systems)
|
|
| Agrobacterium radiobacter Agrobacterium rhizogenes —disarmed strains
Agrobacterium tumefaciens —disarmed strains | 1
non-tumorigenic disarmed Ti plasmid vectors, or Ri plasmid vectors 2
none (non-vector systems) |
|
| Lactobacillus Lactococcus lactis
Oenococcus oeni syn. Leuconostoc oeni Pediococcus Photobacterium angustum
Pseudoalteromonas tunicata Rhizobium (including the genus Allorhizobium )
Sphingopyxis alaskensis syn. Sphingomonas alaskensis Streptococcus
thermophilus Synechococcus —specified strains: (a) PCC
7002 (b) PCC 7942 (c) WH 8102 Synechocystis
species — strain PCC 6803 Vibrio cholerae CVD103-HgR | 1
non-conjugative plasmids 2 none (non-vector systems) |
| 2 |
fungi | Kluyveromyces lactis Neurospora crassa —laboratory strains
Pichia pastoris Saccharomyces cerevisiae Schizosaccharomyces pombe
Trichoderma reesei Yarrowia lipolytica | 1 all vectors 2
none (non-vector systems) |
| 3 | slime moulds |
Dictyostelium species | 1 dictyostelium shuttle vectors,
including those based on the endogenous plasmids Ddp1 and Ddp2 2
none (non-vector systems) |
| 4 | tissue culture | any of the
following if they cannot spontaneously generate a whole animal: (a)
animal or human cell cultures (including packaging cell lines) (b)
isolated cells, isolated tissues or isolated organs, whether
animal or human (c) early non-human mammalian embryos cultured
in vitro | 1 non-conjugative plasmids 2
non-viral vectors, or replication defective viral vectors unable to transduce
human cells 3 baculovirus ( Autographa californica nuclear
polyhedrosis virus), polyhedrin minus 4 none (non-vector
systems) |
|
| either of the following if they are not intended, and are
not likely without human intervention, to vegetatively propagate, flower or
regenerate into a whole plant: (a) plant cell cultures (b)
isolated plant tissues or organs | 1
non-tumorigenic disarmed Ti plasmid vectors, or Ri plasmid vectors, in
Agrobacterium tumefaciens , Agrobacterium radiobacter or Agrobacterium
rhizogenes 2 non-pathogenic viral vectors 3 none
(non-vector systems) |
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